The wound healing effect of herbal ointments formulated with tannin fraction of Ficus racemosa embedded in ointment bases has been evaluated in vivo using the excision wound healing model, on rats. The tannin fraction was extracted by using the standard procedure for separation of tannins. The semisolid herbal Formulations containing 5% and 10% tannin fraction were prepared and applied topically three times a day to open wounds for 14 days post-operatively and compared with base control. The formulation containing the most active phytoconstituent tannins was packed in air tight bottle and evaluated for its stability. The ointment was stored at 00 ,300, 450 at different predetermined intervals i.e. 30, 60 and 90 days and was evaluated for its appearance, spreadability, texture, pH and phase separation. .There was no change in color, pH and no phase separation at 00 and 300 for 90 days. The pH of the ointment was found to be in the range of 5.95-6.36 .The spreadability of the formulation was found to lie between 33.92- 52.77 and the spreading time ranges between 18 sec to 28 sec. The texture of the ointment remained smooth through out the stability studies. The treated wounds showed a faster rate of wound contraction compared with controls. The wound contraction studies revealed that the wound contractions increase with an increase in the herbal extract concentration. The semisolid formulations produced better healing compared with the ointment bases. The results obtained were encouraging semisolid formulations produced better wound healing.
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